P2859
Sigma
Monoclonal Anti-p300/CBP antibody produced in mouse
~2 mg/mL, clone NM11, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-CREB Binding Protein |
| MDL number: | MFCD02097119 |
Description
| Immunogen | native human p300. |
| Specificity | Reacts specifically with both p300 and CBP but not with the related p270 molecule. The epitope recognized by the antibody resides within the 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of the antibody. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | NM11 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 300 kDa |
| species reactivity | human, mink, monkey, rat, mouse |
| concentration | ~2 mg/mL |
| application(s) | immunoblotting1,2: 10-20 μg/mL using human 293 embryonal kidney cells |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation1,2: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... KAT2B(8850) |
| rat ... Pcaf(301164) | |
| mouse ... Kat2b(18519) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Gressner, A., et al., Pharmacological application of caffeine inhibits TGF-β-stimulated connective tissue growth factor expression in hepatocytes via PPARγ and SMAD2/3-dependent pathways. J. Hepatol. 49, 758-767, (2008) Abstract |
